In the process of using the water quality coliform group online analyzer to detect coliform group in the water supply, the user has a reverse tube operation! One of the steps is, when lactose fermentation is tested, there’s a culture process. So at the beginning of the culture, there is a small tube of gas in the small tube, so is it a liquid or a gas? Here’s the explanation:

1.The purpose of letting the small pipe down is to see whether it produces gas after cultivation. The small inverted tube can not be put into the bubble, one is put in if there is a bubble is certainly not possible, you can put the small inverted tube first, in the medium.

If you are not assured, you can test it first and see which method is easy to operate and will not have bubbles. After the small pour tube is clear and clean, the problem is not big after adding, the air inside the small pour tube can be drained after sterilization, in this process, the first put and then put to sterilize, and then look at the effect.

2.If there is gas in the inverted tube, it means that sterilization is not controlled, which has nothing to do with pouring the medium or releasing the small inverted tube first, pay attention to the time of sterilization, when the time is up, you can not be anxious to vent the gas, and wait for the temperature and pressure to drop naturally, then take it, in addition, when sterilization, the tube is tilted at a certain Angle to put the sterilization pot, which also helps exhaust!